Imigqa yeseli ehlelwe ngofuzo
Imigqa yeseli ehlelwe ngofuzo ibhekisela kumaseli athole ukuguqulwa kofuzo ngenqubo eyaziwa ngokuthi ukuhlela izakhi zofuzo.Ukuhlela izakhi zofuzo kubandakanya ukwenza izinguquko ku-DNA yento ephilayo, ngokuvamile ngenjongo yokutadisha izakhi zofuzo ezithile noma ukuqonda izinqubo ezithile zebhayoloji.
Ngokusebenzisa ubuchwepheshe bokuhlela izakhi zofuzo, kungenzeka ukufeza imigqa yamaseli okusebenzisa izakhi zofuzo, imigqa yeseli ephumayo, ukuguqulwa kwamaphoyinti, noma imigqa yamangqamuzana angena ngaphakathi kwamangqamuzana athile, angasetshenziswa ukutadisha ukusebenza kofuzo, ukusayina, izindlela zezifo. , nokuthuthukiswa kwezidakamizwa, kanye nokulebula amaseli athile.
Kunezizathu ezimbalwa zokuthi kungani imigqa yamaseli ahlelwe ngofuzo ibalulekile ocwaningweni lwesayensi.Okokuqala, banikeza ithuluzi lokufunda umsebenzi wezakhi zofuzo ezithile.Ngokushintsha izakhi zofuzo endaweni elawulwayo, ososayensi bangabona umphumela wokuguqulwa kwezakhi zofuzo ekuziphatheni kwamangqamuzana noma ezinye izinqubo zezinto eziphilayo.Lolu lwazi lungaba nomthelela ekuqondeni kangcono izifo zofuzo, kanye nokuhlonza izinhloso zokwelapha ezingase zibe khona.
Ukwengeza, imigqa yeseli ehlelwe ngofuzo ingasetshenziswa ukuthuthukisa nokuhlola imithi emisha.Ngokwethula izinguquko ezithile zofuzo, ososayensi bangakha imigqa yamaseli elingisa izimo zesifo, okubenza bakwazi ukuhlola ukusebenza kwezindlela zokwelapha ezingase zibe khona.Lokhu kuvumela izinqubo zokuthuthukiswa kwezidakamizwa ezinembe kakhudlwana nezisebenza ngempumelelo.
Ngaphezu kwalokho, imigqa yamaseli ahlelwe ngofuzo inamandla okusetshenziswa emithini yokuvuselela kabusha.Ngokushintsha izakhi zofuzo ezinesibopho sokuziphatha kwamangqamuzana noma ukuhlukaniswa, ososayensi bangathuthukisa amandla okwelapha ama-stem cells noma bakhe amaseli afaneleka kakhulu ukufakelwa kabusha.
Inzuzo
1. I-multi-locus ehlelwe ngofuzo ngesikhathi esisodwa;
2. ukuhlinzeka ngesevisi yokuma okukodwa kusuka kumklamo we-sgRNA kanye nokuhlanganisa kuya ekuhlolweni komugqa weselula;
3. Okuhlangenwe nakho okucebile ekuhlanganiseni kwezakhi zofuzo ukusiza ama-vector wokuhlela ufuzo ukusuka ekwakhiweni kwesikimu kuye ekuqedeni kokwakhiwa.
Ibhizinisi Eliyinhloko
- I-Gene Knock-out Cell Lines
- Gene Activation Cell Lines
- Khomba imigqa yeseli ye-mutant
- Knock-in Cell Lines
Izithenjwa
[1]U-Zhang S, u-Shen J, u-Li D, u-Cheng Y. Amasu ekulethweni kwe-Cas9ribonucleoprotein yokuhlela i-CRISPR/Cas9 genome.I-Theranostics.2021 Jan1;11(2):614-648.doi: 10.7150/thno.47007.I-PMID: 33391496;I-PMCID: PMC7738854.
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