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Ukwenza ngokwezifiso igundane le-QuickMice™ homozygous ngokushesha
Iseli kuthiwa liyi-homozygous yofuzo oluthile lapho ama-allele afanayo ofuzo ekhona kuwo womabili ama-chromosome angama-homologous.
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I-QuickMice™ yokwenza ngokwezifiso igundane elenziwe ngofuzo elisheshayo
Amamodeli egundane enziwe abantu anohlu olubanzi lwezinhlelo zokusebenza emikhakheni yocwaningo ye-AIDS, umdlavuza, izifo ezithathelwanayo, nesifo segazi.
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Ukwenza ngokwezifiso igundane kwe-QuickMice™ okusheshayo kwe-KI
I-Knock-in (KI) indlela esebenzisa ukuhlanganiswa kabusha kwe-homologous kwezakhi zofuzo ukuze kudluliselwe isakhi sofuzo esisebenza ngaphandle kwesakhi esilandelanayo kuseli ne-genome, futhi sithole ukubonakaliswa kahle kuseli ngemva kokuhlanganiswa kabusha kofuzo.
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Ukwenza ngokwezifiso igundane le-QuickMice™ le-CKO
I-Conditional Knock-out (CKO) iwubuchwepheshe bokukhipha izakhi zofuzo ezithize ezithize ezitholwe ngohlelo lokuhlanganisa lwasendaweni.
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Ukwenza ngokwezifiso igundane le-QuickMice™ enezakhi eziningi zofuzo
Ngokufaka isiceloI-TurboMice™ubuchwepheshe, singakwazi ukuhlola ngokuqondile amangqamuzana e-embryonic stem ngemva kokuhlelwa kofuzo ezinsukwini ezingu-3-5, bese sakhe iseli ye-tetraploid, futhi sithole amagundane ahlelwe yi-homozygous multi-locus gene-edited ezinyangeni ezingu-3-5 ngemva kokuzala umama wamagundane, angasindisa unyaka ongu-1. kumakhasimende ethu.
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Ukwenza ngokwezifiso igundane elihlelwe yi-QuickMice™ isiqeshana eside
I-TurboMice™ubuchwepheshe buvumela ukuhlela okunembile kofuzo kwezingcezwana ezinde ngaphezu kuka-20kb, ngaleyo ndlela kusize ukukhiqizwa okusheshayo kwamamodeli ayinkimbinkimbi afana nokwenza abantu, i-conditional knockout (CKO), kanye ne-kigment fragment knock-in (KI).