Imininingwane Yomkhiqizo

Omaka bomkhiqizo

Ukwenza Ngokwezifiso Igundane le-Quickmice™ le-CKO

I-Conditional Knock-out (CKO) iwubuchwepheshe bokukhipha izakhi zofuzo ezithize ezithize ezitholwe ngohlelo lokuhlanganisa lwasendaweni.

Enye yezinzuzo ezinkulu ze-conditional knock out yikhono layo lokufunda ukusebenza kofuzo ngendlela elawulwa kakhulu.Ngokukhetha ukwenza izakhi zofuzo zingasebenzi ngezikhathi ezithile noma ezicutshini ezithile, abacwaningi bangakwazi ukubona imiphumela yokulahlekelwa kwezakhi zofuzo futhi bathole ukuqonda mayelana nezinqubo eziyisisekelo zezinqubo ezihlukahlukene zebhayoloji.

Kuthatha isikhathi esingaphezu kwezinyanga eziyi-10-12 ukwakha imodeli yegundane le-CKO ngezinga eliphansi lempumelelo kusetshenziswa uhlelo lwendabuko lwe-Cre/LoxP, njengoba lolu hlelo ludinga amagundane e-Flox ukuthi ahlangane negundane elinesisho esithile se-Cre.

Isizukulwane esisha se-Rapid Mouse Preparation Technology

I-TurboMice™

Singakunikeza ngokushesha amamodeli egundane e-CKO homozygous ngokusebenzisa ubuchwepheshe be-TurboMice™ ukuze sithuthukise izinga lempumelelo.

Ngokusekelwe ohlelweni oluhle kakhulu lokuhlela izakhi zofuzo ososayensi bethu, singaqedela ukuhlolwa kwamaseli e-embryonic stem phakathi kwezinsuku ezingu-3-5, bese sakhe umbungu we-tetraploid.Ngemuva kokubeletha komama, amagundane e-homozygous humanized angatholakala phakathi nezinyanga ezingu-2-4, angasindisa izinyanga ezingu-7-8 kumakhasimende.

Okuqukethwe Kwesevisi

Inombolo Yesevisi. Izinkomba zobuchwepheshe Okuqukethwe kokudiliva Umjikelezo wokulethwa
I-MC003-1 ubude bofuzo olulodwa <5kb 3-9 CKO homozygous amagundane abesilisa 2-4 izinyanga
I-MC003-2 ubude bofuzo olulodwa <5kb 10-19 CKO homozygous amagundane abesilisa 2-4 izinyanga
I-MC003-3 ubude bofuzo olulodwa <5kb 20 CKO amagundane abesilisa homozygous 3-5 izinyanga
I-MC003-4 ubude bofuzo olulodwa buyi-5kb-10kb 3-9 CKO homozygous amagundane abesilisa 3-4 izinyanga
I-MC003-5 ubude bofuzo olulodwa buyi-5kb-10kb 10-19 CKO homozygous amagundane abesilisa 3-4 izinyanga
I-MC003-6 ubude bofuzo olulodwa buyi-5kb-10kb 20 CKO amagundane abesilisa homozygous 3-5 izinyanga

Xhumana nathi

Sicela uxhumane nathi ku:

1) Sicela ulande futhi ugcwalise ifomu elingezansi《Ifomu lesicelo sekhotheshini》, bese uyithumela nge-imeyili kuMingCelerOversea@mingceler.com;

2) Ucingo: +86 181 3873 9432;

3) I-LinkedIn:https://www.linkedin.com/company/ ukuthengar/

Ifomu lesicelo sekhotheshini.docx

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