Ubuchwepheshe Besizukulwane Esilandelayo Esisheshisa Ukudalwa Kwemodeli Yegundane
I-TurboMice™ Technology
Kuthuthukiswe ngabakwa-MingCeler ngochungechunge lokulungiselelwa kwe-tetraploid complementation.
NgokuhlanganisaI-Precise Gene Editing technologykanye negundane elilungiselelweUbuchwepheshe bokulungiselela i-Embryonic Stem Cell, manje sesingakwazi ukuhlela cishe noma iyiphi indawo yofuzo eqondiwe.
I-TurboMice™ Technology
Ubuchwepheshe bethu obusebenza kahle kakhulu bokugcwalisa i-Tetraploid buye busivumela ukuba sikhulise kakhulu izinga lokuzalwa kwamagundane ukusuka ku-1% -5% kuya ku-30% -60% ngamaseli e-stem ahlelwe ngofuzo, acishe afane nokusebenza kahle kokudluliswa kombungu okuvamile.
I-MingCeler iyinkampani yokuqala emhlabeni ukufeza ngempumelelo ukuguqulwa kobuchwepheshe bokugcwalisa i-Tetraploid busuka elabhorethri iye ekusetshenzisweni kwezimboni.
Ubuchwepheshe Bendabuko
Ubuchwepheshe bendabuko bokufanisa izilwane, obufana ne-pronuclear microinjection kanye nokukhomba kwe-ES, kuhlanganisa ukuzalanisa okungenani izizukulwane ezi-2 kuya kwezi-3 ukuze kutholwe amagundane e-homozygous genotype, ngokuvamile ethatha izinyanga eziyisi-6-8.
Le nqubo ende ikhinyabeza kakhulu inqubekelaphambili nokusebenza kahle kokuthuthukiswa kwemishanguzo emisha kanye nocwaningo lwezifo.
Iqoqo Elisheshayo
1. kufika ku-20 amagundane ahlelwe ngofuzo lwe-homozygous ngaphakathi2-4 izinyanga.
2. ≈ 50 amagundane homozygous ngaphakathi kokulandelayo2 izinyanga.
3. 400+ amagundane angama-homozygous ngaphakathi8-12 izinyanga.
Umbiko Wecala ngo-2020
Ubuchwepheshe be-TurboMice™ busetshenziswe ukukhiqiza ngokushesha500 homozygousamagundane e-ACE2 enziwe abantu ngaphakathi nje8 izinyangangezidakamizwa ze-COVID-19 nezifundo zomgomo.
Ukukhetha kwe-Flexible Strain
Amasu endabuko anemikhawulo ebalulekile ekukhetheni uhlobo lwegundane, kuyilapho ubuchwepheshe be-TurboMice™ bunikeza ukuguquguquka okwengeziwe ngezinhlobonhlobo zezinhlobo ezifuywayo neziphumayo ongakhetha kuzo (okuhlanganisa i-Balb /c, ICR, C57BL/6, njll.).
● Amagundane akhiqiza i-F0 akhiwe kusetshenziswa ubuchwepheshe be-TurboMice™ amagundane okuqondiwe kuwo anomsuka weseli elilodwa.
● Ngakho-ke, izakhi zofuzo zamagundane e-F0 ziyefana, zinciphisa amaphutha okuhlola abangelwa ukuhlukahluka kwezakhi zofuzo.
● Ngakho-ke, idatha yokuhlola yokuhlolwa kokusebenza ngempumelelo kwezidakamizwa nokunye ukuhlola kuyahambisana futhi kuthembekile.
Igcina Ubuqotho Bofuzo Oluhle
Ku-Situ Precision Gene Editing
● Ukuhlelwa kofuzo okunembayo okunembayo okunamaleveli anembayo okubonakala kwezakhi zofuzo kanye nezici ezinembile zezicubu.
● Amagundane e-ACE2 enziwe abantu athuthukiswe ngamasu endabuko akhiqizwa ukwethulwa kwangaphandle komgqugquzeli we-K18-ACE2, ongakwazi ukufeza ukuhlela okunembayo ngenxa yokufakwa okungahleliwe, okuholela ekuntulekeni kwezicubu eziqondile zesisho se-ACE2 kumodeli yegundane elakhiwe umuntu le-ACE2. .
● Amagundane e-ACE2 enziwe umuntu we-MingCeler abonisa inkulumo ethize ezithweni ezahlukene (imifanekiso C no-D ngenhla), futhi alingisa ngempumelelo izici zomtholampilo ezilandela ukutheleleka kwe-SARS-CoV-2.
I-Unique EnhancerPlus Platform
●Inkundla ye-EnhancerPlus ephathelene ne-MingCeler ingasiza amakhasimende ethu ukuthi athuthukise amazinga okuveza izakhi zofuzo ezenziwe abantu.
● Umphumela weminyaka yokuqongelelwa kwedatha ku-epigenetics kanye nocwaningo lokunquma isiphetho seseli, inkundla ye-EnhancerPlus ye-MingCeler ingabikezela ngokunembile ukumi kwe-genomic ye-Enhancers, yenze idizayini yamasu okuhlela izakhi zisekelwe ebufakazini.
● Ngenxa yalokho, izakhi zofuzo eziqondiwe zingafana kangcono namaphethini wokukhuluma namaleveli ezakhi zofuzo ezingapheli.
Umbiko wecala: Ukwenziwa komuntu kofuzo lwe-X.
● Ngokusebenzisa inkundla ye-EnhancerPlus, isu lathuthukiswa futhi izinga lenkulumo labona ukukhuphuka kwama-oda amathathu obukhulu (bona umfanekiso ongenhla), kwafinyelela izidingo zokuthuthukiswa kwezidakamizwa njengoba kunqunywa ikhasimende ezingeni leprotheni.
● Ngemva kokuphothula ukukhiqizwa okukhulu komhlaba kwamagundane enziwe abantu i-ACE2 ngo-2020, i-MingCeler ithuthukise imodeli yegundane elenziwe umuntu i-ACE2 ngokuphindaphinda okune ngokusebenzisa i-EnhancerPlus optimization, nezinga lenkulumo ye-ACE2 eyenziwe yaba umuntu ekugcineni ifinyelele eduze kwesisho segundane i-ACE2.
Amazinga wokuvezwa we-ACE2 eyenziwe ngumuntu ezithweni ezihlukene zamamodeli egundane enziwe abantu
I-Multi-Locus Gene Editing
● Ubuchwepheshe be-TurboMice™ buyinketho engcono kakhulu emakethe yokukhiqiza amamodeli amagundane ahlanganisiwe we-multi-locus combinatorial gene, enikeza izinzuzo zokunemba okuphezulu ekufakeni indawo, ukufakwa kwesiqephu esinde, kanye ne-loci eshintshwe izakhi zofuzo eziningi, ngaphandle kwezinkinga zokuhlukaniswa.
● Ubuchwepheshe be-TurboMice™ buvumela ukuhlela kanyekanye kwezakhi zofuzo ezi-3 ukuze kukhiqizwe amamodeli egundane ahlelwe nge-homozygous multi-locus kusuka kumaseli e-embryonic ahlelwa ngofuzo, ngaphandle kwesidingo sezinqubo zokuzala/zokuhlola ezidla isikhathi, okuhlinzeka ngezinhlobonhlobo. yamamodeli ayinkimbinkimbi amanani aphezulu adingekayo ocwaningweni olusha lwezidakamizwa.
● I-MingCeler ithuthukise ngempumelelo amamodeli amagundane ahlelwe ngezakhi zofuzo eziningi ngesikhathi esifushane, ngokusekelwe kumagundane enziwe abantu e-ACE2.
Ukuhlelwa Kwezakhi Zofuzo Ezinde
Ubuchwepheshe be-TurboMice™ buvumela ukuhlela kofuzo okunembayo kwezingcezu ezinde ezingaphezu kuka-20kb, kusiza ukukhiqizwa okusheshayo kwamamodeli ayinkimbinkimbi njenge-humanization, i-conditional knock-out (CKO), kanye ne-gregment fragment knock-in (KI).
Okushicilelwe
[1] Wang G, Yang ML, Duan ZL, Liu FL, Jin L, Long CB, Zhang M, Tang XP, Xu L, Li YC, Kamau PM, Yang L, Liu HQ, Xu JW, Chen JK, Zheng YT , Peng XZ, Lai R. Dalbavancin ibophezela i-ACE2 ukuze ivimbe ukusebenzisana kwayo ne-SARS-CoV-2 spike protein futhi iyasebenza ekuvimbeleni ukutheleleka kwe-SARS-CoV-2 kumamodeli ezilwane.I-Cell Res.2021 Jan;31(1):17-24.doi: 10.1038/s41422-020-00450-0.( IF: 20.507 )
[2] Liu FL, Wu K, Sun J, Duan Z, Quan X, Kuang J, Chu S, Pang W, Gao H, Xu L, Li YC, Zhang HL, Wang XH, Luo RH, Feng XL, Schöler HR , Chen X, Pei D, Wu G, Zheng YT, Chen J. Isizukulwane esisheshayo se-ACE2 imodeli yegundane elenziwe umuntu le-COVID-19 ehambisana ne-tetraploid.I-Natl Sci Rev. 2020 Nov 24;8(2):nwaa285.doi: 10.1093/nsr/nwaa285.( IF: 16.693 )
Ukugeleza Kwesevisi
Odinga ukukwenza nje ukusinikeza isidingo sakho sokuguqulwa kofuzo kumodeli yegundane lakho.Ngokusekelwe ekucaciseni kwakho, sizohlanganisa uhlelo lokuqala olwenziwe ngendlela oyifisayo, futhi ngemva kwengxoxo eyengeziwe nesivumelwano sobabili, sizosayina inkontileka yesevisi yezobuchwepheshe.Ngemva kokuqala kwephrojekthi sizokunikeza izibuyekezo ezifika ngesikhathi ngenqubekelaphambili.